Sepax C-Pro Protocol – NeatCell

Enrich mononuclear cell fractions of apheresis product, bone marrow, and umbilical cord blood via a density gradient medium in combination with the Sepax C-Pro Cell Processing System. Process an initial volume between 30 and 120 mL and select a final volume down to 8 mL.


Key Features


Initial volume


30 to 120 mL


Selectable final volume


8 to 20 mL, or 45 mL


Processing time


~ 1h15 minutes


MNC recovery


> 52%


RBC depletion


> 99%


Step    Details



Prepare a single-use kit with density gradient medium (via microbial filter), washing solution, and a syringe for collecting the final product (if applicable)




Connect initial product to the kit.




Install the kit on the Sepax C-Pro.




The Sepax C-Pro automatically performs the procedure including:
• Priming
• Washing




Collect purified mononuclear cells from the final product bag or via a syringe.

Protocol Information

NeatCell C-Pro protocol software is integral to our Sepax C-Pro Cell Processing System. It allows for an automatic, reproducible, and user-independent procedure. Only for use with compatible Sepax C-Pro equipment.


Compatible Equipment

Sepax C-Pro (#29264741)

Kits: CT-90.1 (#29264740)


Packaging Information

One box for each protocol, including a USB key with the protocol installed and a user installation guide.



Product Specifications

Description Parametres
Product Division Laboratory Division
Brand Cytiva

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Medical Division

Laboratory Division

Industry Business Unit