Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.
The Adipose protocol is designed for routine processing of digested and filtered lipoaspirate, in order to isolate the nucleated cell fraction which is enriched in stem cells. It allows processing of input volumes up to 420 mL, which include raw adipose, collagenase and buffer.
Initial volume | 30 to 420 mL |
Selectable final volume |
8 to 150 mL |
Processing time after |
45 to 60 minutes |
ADSC in POST fraction |
200,000-400,000 cells/mL |
Cell viability |
>90% |
Step | Details |
1 |
Wash and digest collected lipoaspirate. |
2 |
Collect the stromal vascular fraction and attach it to a single-use kit. |
3 |
Install the kit on the Sepax. |
4 |
The Sepax automatically performs the procedure including stem cell isolation and enzyme removal. |
5 |
Extract cells via a syringe or a final product bag. |
Use the separation chamber holder for the last steps of the Adipose procedure when collecting the final cells in a pre-connected syringe.
Kit / protocol | CS-490.1 | CS-470.1 | CS-900.2 | CS-430.1* |
PeriCell | x | – | – | x |
SmartRedux | x | – | – | – |
ReadyCell | – | x | – | – |
NeatCell | – | – | x | – |
Adipose | – | – | x | – |
*Maximum initial volume limited to 600 mL.
Description | Parametres |
---|---|
Product Division | Laboratory Division |
Brand | Cytiva |