Sepax 2 S-100 Protocol – CultureWash

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

CultureWash is designed for washing, exchanging medium, and concentrating cells. It offers flexible parameters and small final volume capabilities when manufacturing cellular therapy products.



Key Features

Features CultureWash

Initial volume


30 to 880 mL


Selectable final volume


8 to 220 mL*


Average CD34+ recovery




Average CD34+ viability



*If product volume reduced to less than 20 mL, CS-900.2 single-use kit and Separation Chamber Holder are needed.


Step Details



Prepare a washing solution.




Prepare a kit with the initial product.




Install the kit on the Sepax.




The Sepax automatically performs the procedure including:
• Primary dilution
• Osmolarity balancing
• Sedimentation and initial product rinsing
• Supernatant extraction
• Cell resuspension
• Cell extraction into a final product bag.

Product features

  • Dilution ratio: Defines the volume of washing solution used to dilute the initial product for osmolarity balancing.
  • Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
  • Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
  • Main user interface available in: English, French, German, Italian, and Spanish.

Kit / Protocol Compatibility Matrix

Kit / protocol CS-600.1 CS-900.2** CS-490.1
CordWash x
SmartWash x
CultureWash x x
Dilution x x x

**The CS-900.2 kit is only validated for initial volumes up to 220 mL. Higher volumes must be validated by the user.


Product Specifications

Description Parametres
Product Division Laboratory Division
Brand Cytiva

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Medical Division

Laboratory Division

Industry Business Unit