Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

CultureWash is designed for washing, exchanging medium, and concentrating cells. It offers flexible parameters and small final volume capabilities when manufacturing cellular therapy products.

 

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Key Features

Features	CultureWash
Initial volume	30 to 880 mL
Selectable final volume	8 to 220 mL*
Average CD34+ recovery	>80%
Average CD34+ viability	95%

*If product volume reduced to less than 20 mL, CS-900.2 single-use kit and Separation Chamber Holder are needed.

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Workflow

Step	Details
1	Prepare a washing solution.
2	Prepare a kit with the initial product.
3	Install the kit on the Sepax.
4	The Sepax automatically performs the procedure including: • Primary dilution • Osmolarity balancing • Sedimentation and initial product rinsing • Supernatant extraction • Cell resuspension • Cell extraction into a final product bag.

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Product features

• Dilution ratio: Defines the volume of washing solution used to dilute the initial product for osmolarity balancing.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: English, French, German, Italian, and Spanish.

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Kit / Protocol Compatibility Matrix

Kit / protocol	CS-600.1	CS-900.2**	CS-490.1
CordWash	x	–	–
SmartWash	x	–	–
CultureWash	x	x	–
Dilution	x	x	x

**The CS-900.2 kit is only validated for initial volumes up to 220 mL. Higher volumes must be validated by the user.

 

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