Sepax 2 S-100 Protocol – CultureWash

https://cytomed.ae/products/sepax-2-s-100-protocol-culturewash/

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

CultureWash is designed for washing, exchanging medium, and concentrating cells. It offers flexible parameters and small final volume capabilities when manufacturing cellular therapy products.

 

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Key Features

Features CultureWash
 

Initial volume

 

30 to 880 mL

 

Selectable final volume

 

8 to 220 mL*

 

Average CD34+ recovery

 

>80%

 

Average CD34+ viability

 

95%

*If product volume reduced to less than 20 mL, CS-900.2 single-use kit and Separation Chamber Holder are needed.


Workflow

Step Details
 

1

 

Prepare a washing solution.

 

2

 

Prepare a kit with the initial product.

 

3

 

Install the kit on the Sepax.

 

4

 

The Sepax automatically performs the procedure including:
• Primary dilution
• Osmolarity balancing
• Sedimentation and initial product rinsing
• Supernatant extraction
• Cell resuspension
• Cell extraction into a final product bag.


Product features

  • Dilution ratio: Defines the volume of washing solution used to dilute the initial product for osmolarity balancing.
  • Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
  • Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
  • Main user interface available in: English, French, German, Italian, and Spanish.

Kit / Protocol Compatibility Matrix

Kit / protocol CS-600.1 CS-900.2** CS-490.1
CordWash x
SmartWash x
CultureWash x x
Dilution x x x

**The CS-900.2 kit is only validated for initial volumes up to 220 mL. Higher volumes must be validated by the user.

 


Product Specifications

Description Parametres
Product Division Laboratory Division
Brand Biosafe Cytiva

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Medical Division

Laboratory Division

Industry Business Unit