Sepax 2 S-100 Protocol – NeatCell

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

NeatCell is a protocol designed for isolation by means of a density gradient of the mononuclear cell (MNC) fraction from various cellular products into a small final volume of 8-20 mL. Alternatively, a fixed final volume of 45 mL can be chosen. In both cases, optimal granulocyte and Red Blood Cells (RBC) depletion is achieved.




Key Features

NeatCell Features

Initial volume


30 to 120 mL


Selectable final volume


8 to 20 mL, or 45 mL


Processing time


~1h15 minutes


MNC recovery


> 52%


RBC depletion


> 99%


Step Details



Prepare a kit with density gradient medium (via microbial filter), washing solution, and a syringe for collecting the final product (if applicable).




Connect initial product to the kit.




Install the kit on the Sepax.




The Sepax automatically performs the procedure including:
• Priming
• Isolation via a density gradient medium
• Washing




Collect purified mononuclear cells from the final product bag or via a syringe.

Product Features

  • Automated procedure comprising of all required steps.
  • Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
  • Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
  • Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

Accessory for small final volume option

Use the separation chamber holder for the last steps of the procedure when collecting the final cells in a pre-connected syringe.

Kit / Protocol Compatibility Matrix

Kit / protocol CS-490.1 CS-470.1 CS-900.2 CS-430.1*
PeriCell x x
SmartRedux x
ReadyCell x
NeatCell x
Adipose x

*Maximum initial volume limited to 600 mL.



Product Specifications

Description Parametres
Product Division Laboratory Division
Brand Cytiva

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Medical Division

Laboratory Division

Industry Business Unit