Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

NeatCell is a protocol designed for isolation by means of a density gradient of the mononuclear cell (MNC) fraction from various cellular products into a small final volume of 8-20 mL. Alternatively, a fixed final volume of 45 mL can be chosen. In both cases, optimal granulocyte and Red Blood Cells (RBC) depletion is achieved.

 

 

---

Key Features

NeatCell Features
Initial volume	30 to 120 mL
Selectable final volume	8 to 20 mL, or 45 mL
Processing time	~1h15 minutes
MNC recovery	> 52%
RBC depletion	> 99%

---

Workflow

Step	Details
1	Prepare a kit with density gradient medium (via microbial filter), washing solution, and a syringe for collecting the final product (if applicable).
2	Connect initial product to the kit.
3	Install the kit on the Sepax.
4	The Sepax automatically performs the procedure including: • Priming • Isolation via a density gradient medium • Washing
5	Collect purified mononuclear cells from the final product bag or via a syringe.

---

Product Features

• Automated procedure comprising of all required steps.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

Accessory for small final volume option

Use the separation chamber holder for the last steps of the procedure when collecting the final cells in a pre-connected syringe.

---

Kit / Protocol Compatibility Matrix

Kit / protocol	CS-490.1	CS-470.1	CS-900.2	CS-430.1*
PeriCell	x	–	–	x
SmartRedux	x	–	–	–
ReadyCell	–	x	–	–
NeatCell	–	–	x	–
Adipose	–	–	x	–

*Maximum initial volume limited to 600 mL.

 

---