Sepax 2 S-100 Protocol – NeatCell

https://cytomed.ae/products/sepax-2-s-100-protocol-neatcell/

Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

NeatCell is a protocol designed for isolation by means of a density gradient of the mononuclear cell (MNC) fraction from various cellular products into a small final volume of 8-20 mL. Alternatively, a fixed final volume of 45 mL can be chosen. In both cases, optimal granulocyte and Red Blood Cells (RBC) depletion is achieved.

 

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Key Features

NeatCell Features
 

Initial volume

 

30 to 120 mL

 

Selectable final volume

 

8 to 20 mL, or 45 mL

 

Processing time

 

~1h15 minutes

 

MNC recovery

 

> 52%

 

RBC depletion

 

> 99%


Workflow

Step Details
 

1

 

Prepare a kit with density gradient medium (via microbial filter), washing solution, and a syringe for collecting the final product (if applicable).

 

2

 

Connect initial product to the kit.

 

3

 

Install the kit on the Sepax.

 

4

 

The Sepax automatically performs the procedure including:
• Priming
• Isolation via a density gradient medium
• Washing

 

5

 

Collect purified mononuclear cells from the final product bag or via a syringe.


Product Features

  • Automated procedure comprising of all required steps.
  • Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
  • Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
  • Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

Accessory for small final volume option

Use the separation chamber holder for the last steps of the procedure when collecting the final cells in a pre-connected syringe.


Kit / Protocol Compatibility Matrix

Kit / protocol CS-490.1 CS-470.1 CS-900.2 CS-430.1*
PeriCell x x
SmartRedux x
ReadyCell x
NeatCell x
Adipose x

*Maximum initial volume limited to 600 mL.

 


 

Product Specifications

Description Parametres
Product Division Laboratory Division
Brand Biosafe Cytiva

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Medical Division

Laboratory Division

Industry Business Unit