Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.
UCB-HES is a protocol designed for concentrating the nucleated cell fraction from Umbilical Cord Blood (UCB) into a selectable final volume of 20-50 mL using Hydroxyethyl Starch (HES) sedimentation agent.
The sedimentation profile of the UCB-HES protocol has been designed to deliver high performances for a wide range of initial product characteristics, with reproducible final volumes.
UCB-HES features | |
Initial volume | 35 to 320 mL |
Selectable final volume | 20 to 50 mL |
Processing time | ~ 35 minutes |
Average TNC recovery | 85 to 99 % |
Average CD34+ recovery | 90 to 99% |
Step | Details |
1 | Prepare a kit with the initial product. |
2 | Install the kit on the Sepax. |
3 | The Sepax automatically performs the procedure. |
3 | Collect the final product. |
Kit / protocol | CS-530.1 | CS-530.4/.4b | CS-540.4/.4b | CS-570.4 | CS-570.1m | CS-570.4mb | CS-570.3m | CS-430.1 |
UCB | x | x | x | x | x | x | x | x |
UCB-HES | x | x | x | x | x | x | x | x |
Description | Parametres |
---|---|
Product Division | Laboratory Division |
Brand | Cytiva |