Our protocol software provides a fully-automated method to separate cellular product into its components in combination with our Sepax Cell Separation System. It enables a reproducible and user independent procedure.

UCB-HES is a protocol designed for concentrating the nucleated cell fraction from Umbilical Cord Blood (UCB) into a selectable final volume of 20-50 mL using Hydroxyethyl Starch (HES) sedimentation agent.

The sedimentation profile of the UCB-HES protocol has been designed to deliver high performances for a wide range of initial product characteristics, with reproducible final volumes.

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Key Features

UCB-HES features
Initial volume	35 to 320 mL
Selectable final volume	20 to 50 mL
Processing time	~ 35 minutes
Average TNC recovery	85 to 99 %
Average CD34+ recovery	90 to 99%

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Workflow

Step	Details
1	Prepare a kit with the initial product.
2	Install the kit on the Sepax.
3	The Sepax automatically performs the procedure.
3	Collect the final product.

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Product Features

• Use both mono stopcock (reduced) or three stopcock (standard) kits.
• Separation profile customized to each cords’ unique characteristics.
• Traceability IDs: Use the Sepax traceability kit (barcode reader + printer) to track specific identification criteria.
• Summary print-out: Print reports at the end of procedures with the Sepax traceability kit (barcode reader + printer).
• Main user interface available in: Chinese, English, French, German, Italian, and Spanish.

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Kit / Protocol Compatibility Matrix

Kit / protocol	CS-530.1	CS-530.4/.4b	CS-540.4/.4b	CS-570.4	CS-570.1m	CS-570.4mb	CS-570.3m	CS-430.1
UCB	x	x	x	x	x	x	x	x
UCB-HES	x	x	x	x	x	x	x	x

 

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