Sepax C-Pro Protocol – CultureWash

Wash, concentrate, and exchange medium of cellular products in combination with the Sepax C-Pro Cell Processing System. Process an initial volume between 30 and 880 mL and select a final volume between 8 and 200 mL.


Key Features


Initial volume


30 to 880 mL*


Selectable final volume


8 to 200 mL**


Average CD34+ recovery


> 80%


Average CD34+ viability


> 95%

*The CT-90.1 kit is only validated for initial volumes up to 220 mL. Higher volumes must be validated by the user.

**CT-90.1 kit required if final volume less than 20 mL



Step Details



Prepare a washing solution.




Prepare a single-use kit with the initial product.




Install the kit on the Sepax C-Pro.




The Sepax C-Pro automatically performs the procedure including:
• Primary dilution
• Osmolarity balancing
• Sedimentation and initial product rinsing
• Supernatant extraction
• Cell resuspension
• Cell extraction into a final product bag.

Protocol Information

CultureWash C-Pro protocol software is integral to our Sepax C-Pro Cell Processing System. It allows for an automatic, reproducible, and user-independent procedure. Only for use with compatible Sepax C-Pro equipment.


Compatible equipment

Sepax C-Pro (#29264741)

Kits: CT-60.1 (#29264739), CT-90.1 (#29264740)


Packaging information

One box for each protocol, including a USB key with the protocol installed and a user installation guide.



Product Specifications

Description Parametres
Product Division Laboratory Division
Brand Cytiva

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Medical Division

Laboratory Division

Industry Business Unit