SeCore kits are high-resolution Human Leukocyte Antigens (HLA) typing products based on sequence based typing. Our kits are designed for ease of use and yield high quality results.
Locus Sequencing Kits provide reagents for amplification, purification, and Sanger sequencing of HLA alleles (variants) from DNA. Sequencing fragments produced using this CE Marked kit are subjected to capillary electrophoresis.
Made to order SeCore GSSP’s, the kits are intended for the identification and definition of Class I and II HLA for research applications. The SeCore HLA Sequencing and GSSP kits provide human histocompatibility information of HLA Class I and Class II Loci using genomic DNA isolated from whole blood specimens.
The SeCore Locus Sequencing Kit provides reagents for amplification, purification, and Sanger sequencing of HLA Locus alleles (variants) from DNA.
Using the Sanger sequencing method, SeCore SBT sequencing kits provide bi-directional sequencing for Class I loci: HLA-A (exons 1 – 5), HLA-B (exons 1a – 5), and HLA-C (exons 1 – 7), and bi-directional sequencing for Class II loci: HLA-DRB1b (exons 2 – 3), HLA-DQB1 (exons 2 – 3), and HLA-DPB1c (exons 2 – 4). The uTYPE™ HLA Sequence Analysis Software is used to evaluate the results against the IMGT/HLA database and to help determine the molecular typing.
a. SeCore B locus kits are unidirectional in exon 1 (1F) only
b. SeCore DRB1 locus kits contain additional sequencing primers for codon 86
c. SeCore DPB1 locus kits contain additional sequencing primers for codon 8 & codon 85
We have a variety of ancillary product and reagents optimized for use with SeCore products. The DNA Wipe Test is used to monitor DNA contamination that may interfere with the accurate reporting of SeCore SBT test results. The test is designed to detect a majority of previously amplified, and non-amplified, human genomic DNA containing sequences specific to exon 2 and/or exon 3 of HLA Class I and Class II genes.